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We present a stretchable substrate integrated with pneumatic actuators,
specially designed to be able to demonstrate the change of intracellular
calcium ion concentration induced by mechanical stress.The intracellular
calcium ion concentration has been measured using human foreskin
fibroblasts. We obtained the fluorescence images of intracellular Ca2+ after
supplying the areal strain of 8.79% for 3s. The area having fluorescence
intensity of larger than 0.6 shows 2.3 times increases. Normalized
fluorescence difference, ĢF/F, increases in the stretched cells, showing
the peak value of 28.1% after 5s from the force application. Therefore,
the developed stretchable platform for cell culture shows the potential
feasible application to monitor mechanical stress dependent
intracellular responses.
References :
Y. J. Heo, Eiji Iwase, Kiyoshi Matsumoto and Isao Shimoyama, "Stretchable Substrates for the Measurement of Intracellular Calcium
Ion Concentration Responding to Mechanical Stress,"
Proceedings of the 22nd IEEE International Conference on Micro Electro Mechanical Systems (MEMS '09), pp. 68-71, Sorrento, Italy, January 25-29, 2009.
[Proceedings]
Y. J. Heo, Eiji Iwase, Kiyoshi Matsumoto and Isao Shimoyama, "Stretchable Substrate Using Pneumatic Actuators for Monitoring
Mechanical-Stress-Dependent Cell Response,"
Proceedings of MicroTAS , pp. 579-581, San Diego, USA, October 12-16, 2008.
Y. J. Heo, Eiji Iwase, Kiyoshi Matsumoto and Isao Shimoyama, "Stretchable
Micropatterned Substrates Using Pneumatic Actuators for Studying
Mechanical-Stress-Dependent Cell Response,"
Proceedings of Proc. of 4th Asia Pacific Conference on Transducers and Micro/Nano Technologies (APCOT '08), June 22-25, 2008.
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