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Stretchable Substrates for the Measurement of
Intracellular Calcium Ion Concentration Responding to Mechanical Stress

yunjung1
 

We present a stretchable substrate integrated with pneumatic actuators, specially designed to be able to demonstrate the change of intracellular calcium ion concentration induced by mechanical stress.The intracellular calcium ion concentration has been measured using human foreskin fibroblasts. We obtained the fluorescence images of intracellular Ca2+ after supplying the areal strain of 8.79% for 3s. The area having fluorescence intensity of larger than 0.6 shows 2.3 times increases. Normalized fluorescence difference, ĢF/F, increases in the stretched cells, showing the peak value of 28.1% after 5s from the force application. Therefore, the developed stretchable platform for cell culture shows the potential feasible application to monitor mechanical stress dependent intracellular responses.

References :
Y. J. Heo, Eiji Iwase, Kiyoshi Matsumoto and Isao Shimoyama, "Stretchable Substrates for the Measurement of Intracellular Calcium Ion Concentration Responding to Mechanical Stress," Proceedings of the 22nd IEEE International Conference on Micro Electro Mechanical Systems (MEMS '09), pp. 68-71, Sorrento, Italy, January 25-29, 2009. [Proceedings]

Y. J. Heo, Eiji Iwase, Kiyoshi Matsumoto and Isao Shimoyama, "Stretchable Substrate Using Pneumatic Actuators for Monitoring Mechanical-Stress-Dependent Cell Response," Proceedings of MicroTAS , pp. 579-581, San Diego, USA, October 12-16, 2008.

Y. J. Heo, Eiji Iwase, Kiyoshi Matsumoto and Isao Shimoyama, "Stretchable Micropatterned Substrates Using Pneumatic Actuators for Studying Mechanical-Stress-Dependent Cell Response," Proceedings of Proc. of 4th Asia Pacific Conference on Transducers and Micro/Nano Technologies (APCOT '08), June 22-25, 2008.