In this paper, we propose a multi-functional probe for recording biological activities in a living single neural cell. An active and simultaneous measurement method using a glass micro injector as a multi-functional probe was examined. A measurement region was estimated to be submicron order by using FDTD simulation. A prototype device was applied to a single neural cell in a buccal ganglion of Aplysia. Since active potentials and calcium concentration reactions induced by electric stimuli were recorded simultaneously, validity of our measurement method was confirmed.

References :
Sumito Nagasawa, Isao Shimoyama, “Calcium Measurement Method by Fluorescent Analyses Using a Glass Micro Injector,” The 12th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers '03), pp. 111-114, Boston, Massachusetts, USA, Jun. 8-12, 2003. [Proceedings]

Sumito Nagasawa, Isao Shimoyama, “Calcium Concentration Measurement by Local Fluorescent-Dye Injection,” Sensors and Actuators B, vol. 102, pp. 7-13, 2004. [Paper]

Sumito Nagasawa, Hidekazu Arai, Ryohei Kanzaki, Isao Shimoyama, “Integrated multi-Functional Probe for Active Measurements in a Single Neural Cell,” The 13th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers '05), pp. 1230-1233, Seoul, Korea, Jun. 5-9, 2005. [Proceedings]