Sub-Micron Aperture Plate for Intracellular Calcium Transient Mesurement


 

We have developed a sub-microscopic fluorescent measurement device. This device is composed of a plate with a sub-micron aperture array and a cell-cultivation chamber. Sub-micron apertures were fabricated on a cover slip using EB lithography and a lift-off method. The cultivation chamber made of silicone elastomer was fabricated by a molding method. We fabricated a 200 nm aperture at the minimum size. Nerve cells from silkworm moth (bombyx mori) were successfully cultured directly on this device. A fluorescent measurement experiment was carried out using this device. We measured calcium influx into a nerve cell induced by an ionomycin stimulus. The change of fluorescent intensity reached 50 %. We confirmed the fluorescent measurement of the device.

References :
Tetsuo Kan, Kiyoshi Matsumoto, Isao Shimoyama, “Cell-Culture Plate with Sub-micron Aperture Array for Near-Field Fluorescent Measurement,” 17th IEEE International Conference on Micro Electro Mechanical Systems (MEMS '04), pp. 399-402, Maastricht, The Netherlands, Jan. 25-29, 2004. [Proceedings]

Tetsuo Kan, Kiyoshi Matsumoto, Isao Shimoyama, “Sub-Micron Aperture Plate for Intracellular Calcium Transient Mesurement,” The 13th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers '05), pp. 1306-1309, Seoul, Korea, Jun. 5-9, 2005. [Proceedings]

Tetsuo Kan, Kazunori Hoshino, Kiyoshi Matsumoto, Isao Shimoyama, “Measurement of Light Intensity Field with a Fluorescent Bead Cantilever,” 19th International Conference on Micro Electro Mechanical Systems (MEMS '06), pp. 822-825, Istanbul, Turkey, January 22-26, 2006. [Proceedings]